Identification and characterization of novel Staphylococcus aureus pathogenicity islands encoding staphylococcal enterotoxins originating from staphylococcal food poisoning isolates
نویسندگان
چکیده
منابع مشابه
Identification of Genes Encoding Classical Staphylococcal Enterotoxins in Staphylococcus Aureus Isolated from Raw Milk
This study describes a multiplex PCR assay developed for the detection of Staphylococcus aureus enterotoxin types: SEA, SEB, SEC, SED, and SEE presented in the literature as classical. The method was then used to analyse the presence of genes encoding these enterotoxins in .S aureus strains isolated from raw milk. A total of 237 raw milk samples were used in the study and 77 (32.5%) of them wer...
متن کاملFood Poisoning and Staphylococcus aureus Enterotoxins
Staphylococcus aureus produces a wide variety of toxins including staphylococcal enterotoxins (SEs; SEA to SEE, SEG to SEI, SER to SET) with demonstrated emetic activity, and staphylococcal-like (SEl) proteins, which are not emetic in a primate model (SElL and SElQ) or have yet to be tested (SElJ, SElK, SElM to SElP, SElU, SElU2 and SElV). SEs and SEls have been traditionally subdivided into cl...
متن کاملCharacterization and expression analysis of Staphylococcus aureus pathogenicity island 3. Implications for the evolution of staphylococcal pathogenicity islands.
We describe the complete sequence of the 15.9-kb staphylococcal pathogenicity island 3 encoding staphylococcal enterotoxin serotypes B, K, and Q. The island, which meets the generally accepted definition of pathogenicity islands, contains 24 open reading frames potentially encoding proteins of more than 50 amino acids, including an apparently functional integrase. The element is bordered by two...
متن کاملStaphylococcal Enterotoxins
Staphylococcus aureus (S. aureus) is a Gram positive bacterium that is carried by about one third of the general population and is responsible for common and serious diseases. These diseases include food poisoning and toxic shock syndrome, which are caused by exotoxins produced by S. aureus. Of the more than 20 Staphylococcal enterotoxins, SEA and SEB are the best characterized and are also reg...
متن کاملInnovative application of mass spectrometry for the characterization of staphylococcal enterotoxins involved in food poisoning outbreaks.
Staphylococcal poisoning is a common food-borne disease for which immunoassays to detect enterotoxins were developed, but these assays often lead to false diagnoses due to interferences or lack of specificity. Absolute quantitative mass spectrometry was for the first time successfully applied to an investigation of a staphylococcal outbreak due to coconut pearls.
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2015
ISSN: 1364-5072
DOI: 10.1111/jam.12786